大孔树脂纯化嘉宝果叶片多酚及其生物活性和组成分析

Biological Activities and Composition Analysis of Polyphenols in Jaboticaba Leaves Purified with Macroporous Resin

摘要:

为探讨嘉宝果（Myrciaria cauliflora）叶片多酚的分离纯化方法，对4种树脂（NKA-2、NKA-9、HPD-826和HPD-400A）进行了筛选，并分析了其多酚的抗氧化、体外降糖活性和组成成分。结果表明，NKA-9树脂适于嘉宝果叶片多酚纯化，最佳工艺条件为：上样液质量浓度2.00 mg/mL、洗脱液乙醇体积分数70%、上样流速1.0 mL/min、上样量204 mL、洗脱流速0.9 mL/min、洗脱量70 mL。嘉宝果叶多酚纯度可达69.86%。嘉宝果叶片纯化后的多酚抗氧化及α-葡萄糖苷酶抑制活性高于纯化前，但α-淀粉酶抑制活性低于纯化前。HPLC结果表明，嘉宝果叶片中含有杨梅苷、芦丁、金丝桃苷和鞣花酸，其中鞣花酸含量最高[（16.15±0.49）mg/g]。因此，NKA-9树脂适合分离纯化嘉宝果叶片多酚，纯化后的多酚抗氧化及α-葡萄糖苷酶抑制活性增强。

Abstract:

To study the separation and purification of polyphenols from Myrciaria cauliflora leaves, four resins, such as NKA-2, NKA-9, HPD-826 and HPD-400A, were screened, and the antioxidant, in vitro hypoglycemic activities and components of polyphenols were analyzed. The results showed that NKA-9 resin was suitable for the purification of polyphenols from the leaves. The optimum conditions were as follows:sample concentration of 2.00 mg/mL, flow rate at 1.0 mg/mL and sample loading of 204 mL, 70 mL 70% (V/V) ethanol as elution with velocity at 0.9 mL/min. Under the conditions, the purity of polyphenols from the leaves could reach 69.86%. The antioxidant and α-glucosidase inhibitory activities of purified polyphenols from leaves of M. cauliflora were stronger than those of crude polyphenols, but the inhibitory activities on α-amylase were lower. According to HPLC, the monophenols in leaves of M. cauliflora contained myricetin, rutin, hyperoside and ellagic acid, and ellagic acid content was highest with (16.15±0.49) mg/g. Therefore, NKA-9 resin was suitable for separation and purification of polyphenols from leaves of M. cauliflora, the antioxidant and α-glucosidase inhibitory activities of purified polyphenols increased.